{"product_id":"cst-45965t","title":"CST,  45965T, HLTF (E9H5I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SMARCA3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HLTF (E9H5I) Rabbit Monoclonal Antibody (CST #45965) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHLTF (E9H5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total HLTF protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala998 of human HLTF protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHelicase-like transcription factor (HLTF), also known as RUSH-1 or SMARCA3, belongs to the SNF2\/SWI2 family of ATP-dependent remodelers (1-3). HLTF has been shown to interact with ubiquitin enzyme complexes to promote Lys-63-linked polyubiquitination of PCNA, maintaining genomic stability during DNA damage (4,5). HLTF has double-stranded DNA translocation activity, which can restart replication forks at DNA lesions, providing tumor suppressive functions (6). The gene is frequently hypermethylated in many different tumor types (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDNA-binding protein\/plasminogen activator inhibitor 1 regulator; DNA-binding protein\/plasminogen activator inhibitor-1 regulator; helicase like transcription factor; Helicase-like transcription factor; HIP116; HIP116A; HLTF; HLTF1; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; RNF80; RUSH; SMARCA3; SNF2-like 3; SNF2L3; Sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI\/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI\/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; ZBU1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799528853673,"sku":"45965T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45965t","provider":"Iright","version":"1.0","type":"link"}