{"product_id":"cst-4621t","title":"CST,  4621T, Phospho-TrkA (Tyr674\/675)\/TrkB (Tyr706\/707) (C50F3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TrkA (Tyr680\/Tyr681) phosphate\/TrkB (Tyr706\/Tyr707) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TrkA (Tyr674\/675)\/TrkB (Tyr706\/707) (C50F3) Rabbit Monoclonal Antibody (CST #4621) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-TrkA (Tyr674\/675)\/TrkB (Tyr706\/707) (C50F3) Rabbit Monoclonal Antibody detects endogenous levels of TrkA and TrkB only when phosphorylated at Tyr674\/675 of TrkA and Tyr706\/707 of TrkB. This antibody may cross-react with a protein of ~150 kDa phosphorylated at an unknown tyrosine residue.\nSpecies Reactivity: Human, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr674\/675 of human TrkA.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674\/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10). The phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674\/675 of TrkA to Tyr706\/707 in TrkB of the human sequence (14). TrkB is overexpressed in tumors, such as neuroblastoma, prostate adenocarcinoma, and pancreatic ductal adenocarcinoma (15). Research studies have shown that in neuroblastomas, overexpression of TrkB correlates with an unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF) (16-18). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms' tumors and this isoform may act as a dominant-negative regulator of TrkB signaling (17).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBDNF-tropomyosine receptor kinase B; BDNF\/NT-3 growth factors receptor; DKFZp781I14186; EIEE58; gp140trk; GP145-TrkB; High affinity nerve growth factor receptor; MTC; neurotrophic receptor tyrosine kinase 1; neurotrophic receptor tyrosine kinase 2; Neurotrophic tyrosine kinase receptor type 1; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 1; neurotrophic tyrosine kinase, receptor, type 2; NTRK1; NTRK2; OBHD; Oncogene TRK; p140-TrkA; TRK; Trk-A; Trk-B; TRK1; TRK1-transforming tyrosine kinase protein; TRKA; TRKB; TrkB tyrosine kinase; Tropomyosin-related kinase A; Tropomyosin-related kinase B; Tyrosine kinase receptor; tyrosine kinase receptor A; tyrosine kinase receptor B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799530459305,"sku":"4621T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4621t","provider":"Iright","version":"1.0","type":"link"}