{"product_id":"cst-46343sf","title":"CST,  46343SF, Calbindin (D1I4Q) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying Calbindin. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Calbindin (D1I4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #46343) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier-free version of product #13176. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN, or CUT\u0026amp;Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13176 .\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCalbindin (D1I4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total calbindin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse calbindin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCalcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAB27; CALB; CALB1; Calbindin; calbindin 1; calbindin 1, (28kD); calbindin 1, 28kDa; calbindin 27; Calbindin D28; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799531540649,"sku":"46343SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-46343sf","provider":"Iright","version":"1.0","type":"link"}