{"product_id":"cst-4676s","title":"CST,  4676S, AMPA Receptor 3 (GluA 3) (D47E3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GluR3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, AMPA Receptor 3 (GluA 3) (D47E3) Rabbit Monoclonal Antibody (CST #4676) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:2000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAMPA Receptor 3 (GluA 3) (D47E3) Rabbit Monoclonal Antibody detects endogenous levels of total GluA 3 protein. This antibody is not predicted to detect other AMPA receptor subunits (e.g. GluA 1, GluA 2 or GluA 4) based on sequence homology of the antigen.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro590 of human GluA 3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4) that assemble as homo- or hetero-tetramers and mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization and plasticity. Post-transcriptional modifications (alternative splicing and nuclear RNA editing) and post-translational modifications (glycosylation, phoshorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs (1). GluR 3 knockout mice exhibited normal basal synaptic transmission and long-term depression (LTD) but enhanced long-term potentiation (LTP). In contrast, GluR 2\/3 double knockout mice are impaired in basal synaptic transmission (2). Aberrant GluR 3 expression or activity is implicated in a number of diseases, including autoimmune epilepsy, X-linked mental retardation, Rett's syndrome, amyotrophic lateral sclerosis and Alzheimer disease (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GluR-3; GluR-C; GluR-K3; GLUR3; GLURC; glutamate ionotropic receptor AMPA type subunit 3; Glutamate receptor 3; glutamate receptor C; Glutamate receptor ionotropic, AMPA 3; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; GRIA3; MRX94; MRXSW\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799534817449,"sku":"4676S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4676s","provider":"Iright","version":"1.0","type":"link"}