{"product_id":"cst-46993s","title":"CST,  46993S, TCF4\/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated)","description":"Monoclonal Antibody for studying ITF2. Validated for Chromatin IP,CUT\u0026amp;RUN,CUT\u0026amp;Tag. Available in 2 sizes. Highly specific and rigorously validated in-house, TCF4\/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated) (CST #46993) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nChromatin IP: 1:100\nCUT\u0026amp;RUN: 1:100\nCUT\u0026amp;Tag: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Chromatin IP, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTCF4\/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated) recognizes endogenous levels of total TCF4\/ITF2 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4\/ITF2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTranscription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBHLHB19; CDG2T; Class B basic helix-loop-helix protein 19; E2-2; FECD3; Immunoglobulin transcription factor 2; ITF-2; ITF2; MGC149723; MGC149724; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; SL3-3 enhancer factor 2; TCF-4; TCF4; Transcription factor 4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800329015465,"sku":"46993S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-46993s","provider":"Iright","version":"1.0","type":"link"}