{"product_id":"cst-47074s","title":"CST,  47074S, CD162\/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SELPLG. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD162\/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody (CST #47074) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nFlow Cytometry (Live): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD162\/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD162\/PSGL-1 protein. This antibody reacts with monomeric and dimeric forms of CD162\/PSGL-1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly281 of human CD162\/PSGL-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nP-selectin glycoprotein ligand-1 (PSGL-1\/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high affinity interactions between PSGL-1 and multiple selectins, which facilitates immune cell trafficking to primary and secondary lymphoid organs (4-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD162; CLA; cutaneous lymphocyte-associated associated antigen; P-selectin glycoprotein ligand 1; PSGL-1; PSGL1; Selectin P ligand; SELPL; SELPLG\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 240, 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799537930409,"sku":"47074S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47074s","provider":"Iright","version":"1.0","type":"link"}