{"product_id":"cst-4725s","title":"CST,  4725S, p48 Primase (8G10) Rat Monoclonal Antibody","description":"Monoclonal Antibody for studying p48 Primase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, p48 Primase (8G10) Rat Monoclonal Antibody (CST #4725) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np48 Primase (8G10) Rat Monoclonal Antibody detects endogenous levels of total p48 primase protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with full-length recombinant human p48 primase.\n\u003cb\u003eBackground\u003c\/b\u003e\nInitiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes synthesis of an RNA\/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDNA primase 1; DNA primase 49 kDa subunit; DNA primase polypeptide 1; DNA primase small subunit; DNA primase subunit 1; DNA primase subunit 48; MGC12308; p49; PRI1; PRIM1; primase (DNA) subunit 1; primase p49 subunit; primase polypeptide 1, 49kDa; primase, DNA, polypeptide 1 (49kDa)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rat IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799539568809,"sku":"4725S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4725s","provider":"Iright","version":"1.0","type":"link"}