{"product_id":"cst-4730s","title":"CST,  4730S, Pim-2 (D1D2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Pim-2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pim-2 (D1D2) Rabbit Monoclonal Antibody (CST #4730) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPim-2 (D1D2) Rabbit Monoclonal Antibody detects endogenous levels of total Pim-2 protein. This antibody does not cross-react with other Pim family members.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys266 of human Pim-2.\n\u003cb\u003eBackground\u003c\/b\u003e\nPim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine\/threonine kinases (1). Pim-1, a serine\/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). Pim-2 is highly homologous to Pim-1 with similar oncogenic functions (13,14). Three isoforms of Pim-2 can be generated from alternative start sites which run at 34, 38, and 40 kDa (13). Pim-2 leads to resistance to a variety of apoptotic stimuli and its expression is negatively regulated by growth factor withdrawal (15,16). Increased levels of Pim-2 have also been observed in certain cancers (17,18).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\npim-2 oncogene; Pim-2 proto-oncogene, serine\/threonine kinase; Pim-2h; PIM2; proto-oncogene Pim-2 (serine threonine kinase); serine\/threonine protein kinase pim-2; Serine\/threonine-protein kinase pim-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40, 38, 34\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799540420777,"sku":"4730S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4730s","provider":"Iright","version":"1.0","type":"link"}