{"product_id":"cst-47387t","title":"CST,  47387T, PAPD5 (E9A5D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PAPD5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PAPD5 (E9A5D) Rabbit Monoclonal Antibody (CST #47387) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPAPD5 (E9A5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAPD5 protein. This antibody detects 170 and 120 kDa proteins of unknown identity in some cell lines.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly74 of human PAPD5 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPAP-associated domain-containing protein 5 (PAPD5) and PAPD7, also known as TENT4B and TENT4A, respectively, are poly(A) polymerases involved in a layer of mRNA regulation by depositing guanosine residues on the poly(A) tail. This process, called mixed tailing, interferes with the deadenylase CCR4-NOT complex from efficiently deadenylating the poly(A) tail (1). PAPD5 and PAPD7 form a complex with ZCCHC14 and play a role in stabilization of hepatitis RNA (2-4). Small molecule inhibitors of PAPD5 and PAPD7 are being pursued for treatment against hepatitis B by inhibiting their polyadenylase activity (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ40270; GLD4; Non-canonical poly(A) RNA polymerase PAPD5; PAP associated domain containing 5; PAP-associated domain-containing protein 5; PAPD5; poly(A) RNA polymerase D5, non-canonical; TENT4B; Terminal guanylyltransferase; terminal nucleotidyltransferase 4A; Terminal nucleotidyltransferase 4B; Terminal uridylyltransferase 3; Topoisomerase-related function protein 4-2; TRF4-2; TUT3; TUTase 3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 82\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799540813993,"sku":"47387T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47387t","provider":"Iright","version":"1.0","type":"link"}