{"product_id":"cst-47414s","title":"CST,  47414S, Whitlow\/218 Linker (F2G3S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying . Validated for WB,IP,IHC,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Whitlow\/218 Linker (F2G3S) Rabbit Monoclonal Antibody (CST #47414) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\nFlow Cytometry (Live): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99898 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nWhitlow\/218 Linker (F2G3S) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow\/218 linker. This antibody cross-reacts with a protein of unknown identity at approximately 35 kDa in Jurkat cell extracts.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow\/218 linker peptide.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Whitlow\/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow\/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800221864105,"sku":"47414S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47414s","provider":"Iright","version":"1.0","type":"link"}