{"product_id":"cst-47415s","title":"CST,  47415S, CUT\u0026Tag Dual Index Primers and PCR Master Mix for Illumina Systems","description":"CUT\u0026amp;Tag Kit for studying in the research area.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nStore all components at -20ºC. This product is stable for 18 months if stored properly.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThis kit can generate DNA libraries using CUT\u0026amp;Tag DNA. Note: While CUT\u0026amp;Tag DNA libraries generated for histone modifications typically show robust signal in Agilent Bioanalyzer or TapeStation systems analysis, libraries generated for non-histone proteins such as transcription factors and cofactors often have very weak or even no visible signal using Bioanalyzer or TapeStation systems, but still generate NG-sequencing results with high mapping rates, high numbers of identified binding peaks, and decent signal-to-noise ratios across the whole genome.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eBackground\u003c\/b\u003e\nSimilar to Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN), Cleavage Under Targets and Tagmentation (CUT\u0026amp;Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT\u0026amp;Tag has many of the same advantages as the CUT\u0026amp;RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT\u0026amp;Tag assay adds an adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT\u0026amp;RUN assay, free from DNA end repair, A-tailing, and adaptor ligation . CUT\u0026amp;Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBarcode; C\u0026amp;T; cut and tag; cut\u0026amp;tag; CUT\u0026amp;Tag; cutandtag; DNA library; DNA library construction; DNA library preparation; Index; next gen sequencing; NG-sequencing; NGS; Oligo; sequencing","brand":"CST","offers":[{"title":"Default Title","offer_id":46800805822633,"sku":"47415S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47415s","provider":"Iright","version":"1.0","type":"link"}