{"product_id":"cst-4746t","title":"CST,  4746T, TRA-1-60(S) (TRA-1-60(S)) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying TRA-1-60 antigen. Validated for WB,IHC,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TRA-1-60(S) (TRA-1-60(S)) Mouse Monoclonal Antibody (CST #4746) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:800 - 1:1600\nFlow Cytometry (Live): 1:800 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTRA-1-60(S) (TRA-1-60(S)) Mouse Monoclonal Antibody detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.\n\u003cb\u003eBackground\u003c\/b\u003e\nTRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nGCTM-2 antigen; gp135; Gp200; MGC138240; PC; PCLP; PCLP-1; PCLP1; PDX; Podocalyxin; podocalyxin like; podocalyxin-like; Podocalyxin-like protein 1; PODXL; PODXL1; TRA-1-60 antigen; TRA-1-61 antigen; TRA-1-80 antigen; TRA-1-81 antigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 200-400\nSource\/Isotype: Mouse IgM","brand":"CST","offers":[{"title":"Default Title","offer_id":46799541895337,"sku":"4746T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4746t","provider":"Iright","version":"1.0","type":"link"}