{"product_id":"cst-4747t","title":"CST,  4747T, TRA-2-54 (2J) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Alkaline phosphatase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRA-2-54 (2J) Mouse Monoclonal Antibody (CST #4747) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTRA-2-54 (2J) Mouse Monoclonal Antibody recognizes endogenous levels of total TRA-2-54 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with 2102Ep human embryonal carcinoma cells.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver\/bone\/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation (1,2). The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAlkaline phosphatase liver\/bone\/kidney isozyme; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver\/bone\/kidney; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; AP-TNAP; APTNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver\/bone\/kidney-type alkaline phosphatase; MGC161443; MGC167935; PPBT; tissue non-specific alkaline phosphatase; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; TNALP; TNAP; TNSALP\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 80\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799543861417,"sku":"4747T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4747t","provider":"Iright","version":"1.0","type":"link"}