{"product_id":"cst-47677s","title":"CST,  47677S, ARK5 (E4T2A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NUAK1\/Ark5. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, ARK5 (E4T2A) Rabbit Monoclonal Antibody (CST #47677) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:125 - 1:500\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nARK5 (E4T2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARK5 protein. Non-specific staining was observed in mouse testis by immunohistochemistry.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ARK5 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAMP-activated protein kinases (AMPKs) constitute a serine\/threonine protein kinase family, which is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPKα1, AMPKα2, MELK, and SNARK are the catalytic subunits in the family (2). Recently, AMPK-related protein kinase ARK5 was identified, which shares 84% similarity with the sequence of the catalytic domain of SNARK (2). , Akt phosphorylates ARK5 at Ser600. This phosphorylation activates ARK5, resulting in increased phosphorylation of the SAMS peptide, an AMPK consensus substrate (2). experiments showed that Akt-activated ARK5 is critical for the survival of cells under glucose starvation (2). Furthermore, studies also linked ARK5 to tumor invasion (3,4). Overexpressed ARK5 leads to a higher tumor growth rate (3). For example, the overexpression of ARK5 in pancreatic tumor cell line PANC-1 significantly elevates its metastasis in the liver (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; KIAA0537; NUAK family kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; NUAK1; Omphalocele kinase 1; OMPHK1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799554576553,"sku":"47677S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47677s","provider":"Iright","version":"1.0","type":"link"}