{"product_id":"cst-4777t","title":"CST,  4777T, Lamin A\/C (4C11) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Lamin A\/C (full-length). Validated for WB,WB,IP,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Lamin A\/C (4C11) Mouse Monoclonal Antibody (CST #4777) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:2000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34698 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLamin A\/C (4C11) Mouse Monoclonal Antibody detects endogenous levels of lamin A and lamin C proteins. This antibody also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant fragment of human lamin A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A\/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A\/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A\/C; lamin A\/C-like 1; Lamin-A\/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; Prelamin-A\/C; PRO1; Renal carcinoma antigen NY-REN-32\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 74 (Lamin A), 63 (Lamin C)\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46799562309801,"sku":"4777T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4777t","provider":"Iright","version":"1.0","type":"link"}