{"product_id":"cst-47903s","title":"CST,  47903S, CD127\/IL-7R alpha (A7R34) Rat Monoclonal Antibody (FITC Conjugate)","description":"Monoclonal Antibody for studying IL7R mouse. Validated for Immunofluorescence (Frozen),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD127\/IL-7R alpha (A7R34) Rat Monoclonal Antibody (FITC Conjugate) (CST #47903) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 1.0 μg of antibody per test.\nImmunofluorescence (Frozen): 1:200 - 1:400\nFlow Cytometry (Live): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD127\/IL-7R alpha (A7R34) Rat Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total CD127\/IL-7Rα protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe IL-7 receptor (IL-7R) is comprised of two protein subunits, CD127\/IL-7Rα (IL-7Ralpha) and the common gamma chain (CD132), which is the major signaling component for several cytokines including IL-2, IL-4, IL-9, IL-15, and IL-21 (1). CD127\/IL-7Rα is a transmembrane protein belonging to the cytokine receptor homology class 1 (CRH1) and is expressed by a wide variety of cells including immature B cells, thymic natural killer cells, bone marrow stromal cells, and T cells (5-6). On its own, CD127\/IL-7Rα functions as a receptor for two cytokine receptor complex signaling cascades: IL-7 and thymic stromal lymphopoietin (TSLP) (2). IL-7 signaling contributes to T cell development and homeostasis whereas TSLP receptor signaling contributes to dendritic cell activation and B cell development. IL-7 signaling is an essential component in regulating the homeostasis of naive and memory T cells as differential expression of CD127\/IL-7Rα is observed on naive and activated T cells, which occurs following TCR activation. Specifically, CD127\/IL-7Rα expression is downregulated on activated T cells and the subsequent re-expression of CD127\/IL-7Rα on these cells is indicative of cells that will differentiate into memory T cells (3-4). The A7R34 antibody is widely used to identify CD127\/IL-7Rα expression on both B and T cells (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD127; IL-; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL-7Ralpha; Il7r; IL7RA; interleukin 7 receptor; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; MGC107557\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2a kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800411230377,"sku":"47903S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-47903s","provider":"Iright","version":"1.0","type":"link"}