{"product_id":"cst-48105s","title":"CST,  48105S, CUT\u0026RUN 4X Stop Buffer","description":"Buffer for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor the CUT\u0026amp;RUN assay, we recommend adding 37.5 μl CUT\u0026amp;RUN 4X Stop Buffer, 3.75 μl Digitonin Solution # 16359 , and 0.75 μl RNAse A (10 mg\/ml) # 7013 to 108 μl nuclease-free water (150 μl per reaction) right before use. Then incubate with the sample at 37°C for 10 min. An appropriate amount of Sample Normalization Spike-In DNA can be added to CUT\u0026amp;RUN 4X Stop Buffer, if desired.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore CUT\u0026amp;RUN 4X Stop Buffer at -20°C. This product is stable for at least 12 months.\n\u003cb\u003eBackground\u003c\/b\u003e\nLike the chromatin immunoprecipitation (ChIP) assay, Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN) is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-4). CUT\u0026amp;RUN provides a rapid, robust, and true low cell number assay for detection of protein-DNA interactions in the cell. Unlike the ChIP assay, CUT\u0026amp;RUN is free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a much faster and more efficient method for enriching protein-DNA interactions and identifying target genes. CUT\u0026amp;RUN can be performed in less than one day, from live cells to purified DNA, and has been shown to work with as few as 500-1,000 cells per assay (1,2). Instead of fragmenting all of the cellular chromatin as done in ChIP, CUT\u0026amp;RUN utilizes an antibody-targeted digestion of chromatin, resulting in much lower background signal than seen in the ChIP assay. As a result, CUT\u0026amp;RUN requires only 1\/10th the sequencing depth that is required for ChIP-seq assays (1,2). Finally, the inclusion of simple spike-in control DNA allows for accurate quantification and normalization of target-protein binding that is not possible with the ChIP method. This provides for effective normalization of signal between samples and between experiments.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\u0026amp;R; CnR; CUT \u0026amp; RUN; cut and run; CUT and RUN; CUT\u0026amp;RUN; cutandrun; CUTandRUN; histone modification","brand":"CST","offers":[{"title":"Default Title","offer_id":46800804970665,"sku":"48105S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-48105s","provider":"Iright","version":"1.0","type":"link"}