{"product_id":"cst-48453t","title":"CST,  48453T, EEA1 (E9Q6G) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying EEA1. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, EEA1 (E9Q6G) Mouse Monoclonal Antibody (CST #48453) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEEA1 (E9Q6G) Mouse Monoclonal Antibody recognizes endogenous levels of total EEA1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium\/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEarly endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain-containing protein 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 170\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799613198505,"sku":"48453T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-48453t","provider":"Iright","version":"1.0","type":"link"}