{"product_id":"cst-4850t","title":"CST,  4850T, COX IV (3E11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying COX IV. Validated for WB,WB,WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, COX IV (3E11) Rabbit Monoclonal Antibody (CST #4850) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFluorescent Western: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 97783 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCOX IV (3E11) Rabbit Monoclonal Antibody detects endogenous levels of total COX IV protein.\nSpecies Reactivity: Human, Rat, Monkey, Zebrafish, Bovine, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The three largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOX IV-1; COX4; COX4-1; COX41; COX4I1; COXIV; COXIV-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit 4I1; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; MGC72016\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R Mk Z B Pg\nSENSITIVITY: Endogenous\nMW (kDa): 17\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799617982633,"sku":"4850T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4850t","provider":"Iright","version":"1.0","type":"link"}