{"product_id":"cst-48592t","title":"CST,  48592T, GRASP55\/GORASP2 (F8L6I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GRASP55. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GRASP55\/GORASP2 (F8L6I) Rabbit Monoclonal Antibody (CST #48592) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGRASP55\/GORASP2 (F8L6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRASP55\/GORASP2 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val425 of human GRASP55\/GORASP2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nIn unconventional protein secretion (UPS), cytoplasmic cargo proteins that do not have a signal peptide or a transmembrane domain are transported to the plasma membrane or extracellular matrix mainly to respond to cellular stresses (1,2). The Golgi reassembly-stacking protein 2 (GRASP55, also known as GORASP2) is essential to type III and type IV UPS (2,3). Studies show that mTORC1 phosphorylates GRASP55\/GORASP2 and keeps it at the Golgi apparatus under non-stressed conditions. When cells are stressed, mTORC1 is inhibited, leading to dephosphorylation of GRASP55\/GORASP2. Dephosphorylated GRASP55\/GORASP2 relocates to autophagosomes and multivesicular bodies to promote UPS. In addition, Thr264 on GRASP55\/GORASP2 was shown to seemingly play a key role in mTORC1-mediated phosphorylation of the protein (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp434D156; FLJ13139; Golgi phosphoprotein 6; golgi reassembly stacking protein 2; golgi reassembly stacking protein 2, 55kDa; Golgi reassembly-stacking protein 2; Golgi reassembly-stacking protein of 55 kDa; GOLPH6; GORASP2; GORS2; GRASP55; GRS2; p59\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 61\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799626633385,"sku":"48592T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-48592t","provider":"Iright","version":"1.0","type":"link"}