{"product_id":"cst-4864s","title":"CST,  4864S, PTP-PEST (AG10) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying PTP-PEST. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PTP-PEST (AG10) Mouse Monoclonal Antibody (CST #4864) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPTP-PEST (AG10) Mouse Monoclonal Antibody detects endogenous levels of total PTP-PEST protein. This antibody does not cross-react with other protein tyrosine phosphatases.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with human PTP-PEST recombinant protein. The antibody recognizes an epitope within the amino-terminal 305 residues.\n\u003cb\u003eBackground\u003c\/b\u003e\nPTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration, and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASP, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak, and WASP, PTP-PEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substrates such as paxillin, p130 Cas, Crk, and PSTPIP1 (8). PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nprotein tyrosine phosphatase non-receptor type 12; protein tyrosine phosphatase, non-receptor type 12; Protein-tyrosine phosphatase G1; PTN12; PTP-PEST; PTPG1; PTPN12; Tyrosine-protein phosphatase non-receptor type 12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 110 to 125\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799629549737,"sku":"4864S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4864s","provider":"Iright","version":"1.0","type":"link"}