{"product_id":"cst-48789s","title":"CST,  48789S, UPP1 Antibody","description":"Polyclonal Antibody for studying UPP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, UPP1 Antibody (CST #48789) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUPP1 Antibody recognizes endogenous levels of total UPP1 protein. This antibody may also detect a non-specific band at approximately 45 kDa in a limited number of cell lines.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys174 of human UPP1 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nUridine phosphorylases (UPP1, UPP2) are metabolic enzymes that catalyze the reversible phosphorolytic cleavage of uridine into uracil and ribose-1-phosphate, which can subsequently serve as cellular energy sources or as substrates for nucleotide biosynthesis via pyrimidine salvage pathways (1). Altered pyrimidine metabolism has been reported in multiple cancer subtypes (2-5) and is hypothesized to be associated with cellular metabolic reprogramming events that enable enhanced tumor growth and metastasis (6,7). Notably, UPP1 was identified as one of the enzymes involved in the metabolic activation of the anti-neoplastic agent 5-fluorouracil (5-FU) (8,9). Somewhat paradoxically, numerous UPP1 inhibitors have been developed to reduce the adverse side effects of 5-FU treatment by elevating physiological levels of uridine during 5-FU administration (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nUDRPASE; UP; UPASE; UPase 1; UPP; UPP1; UrdPase 1; Uridine phosphorylase 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 33\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799644655785,"sku":"48789S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-48789s","provider":"Iright","version":"1.0","type":"link"}