{"product_id":"cst-4887s","title":"CST,  4887S, Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)","description":"Monoclonal Antibody for studying NFkB-p65 (Ser536) phosphate. Validated for Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 647 Conjugate) (CST #4887) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-NF-kappaB p65 (Ser536) (93H1) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65. The antibody was conjugated to Alexa Fluor 647 under optimal conditions with an F\/P ratio of 2-6. The Alexa Fluor 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.\n\u003cb\u003eBackground\u003c\/b\u003e\nTranscription factors of the nuclear factor κB (NF-κB)\/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105\/p50), and NF-κB2 (p100\/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCMCU; MGC131774; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFkB-p65; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; RELA proto-oncogene, NF-kB subunit; RELA\/p65; TF65; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk Pg\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800413851817,"sku":"4887S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4887s","provider":"Iright","version":"1.0","type":"link"}