{"product_id":"cst-4891s","title":"CST,  4891S, UBE1a\/b Antibody","description":"Polyclonal Antibody for studying UBE1. Validated for Western Blotting. Highly specific and rigorously validated in-house, UBE1a\/b Antibody (CST #4891) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUBE1a\/b Antibody detects endogenous levels of total UBE1a and UBE1b proteins.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr800 of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nA1S9; A1S9T; A1S9T and BN75 temperature sensitivity complementing; A1ST; AMCX1; CFAP124; GXP1; MGC4781; POC20; POC20 centriolar protein homolog; Protein A1S9; SMAX2; testicular secretory protein Li 63; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1X; ubiquitin like modifier activating enzyme 1; Ubiquitin-activating enzyme E1; ubiquitin-like modifier activating enzyme 1; Ubiquitin-like modifier-activating enzyme 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 110, 117\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799655403689,"sku":"4891S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4891s","provider":"Iright","version":"1.0","type":"link"}