{"product_id":"cst-4892s","title":"CST,  4892S, p63-alpha Antibody","description":"Polyclonal Antibody for studying p63. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, p63-alpha Antibody (CST #4892) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np63-alpha Antibody detects endogenous levels of total p63alpha protein. This antibody will detect both full length TAp63alpha and truncated deltaNp63alpha, but not p63beta or p63gamma.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within the carboxy-terminus of p63alpha. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a truncated ΔNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DeltaNp63; EEC3; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p53CP; P63; P73H; P73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; Tumor protein 63; tumor protein p53-competing protein; tumor protein p53-like; tumor protein p63; tumor protein p63 deltaN isoform delta; Tumor protein p73-like\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799655501993,"sku":"4892S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4892s","provider":"Iright","version":"1.0","type":"link"}