{"product_id":"cst-49054t","title":"CST,  49054T, ARID5A (F1X7C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ARID5A mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ARID5A (F1X7C) Rabbit Monoclonal Antibody (CST #49054) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nARID5A (F1X7C) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID5A protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln289 of mouse ARID5A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAT-rich interactive domain-containing protein 5A (ARID5A) is a member of the ARID family, which includes 15 distinct human proteins. ARID is a nucleic acid binding domain that has a helix-turn-helix motif and is conserved in all eukaryotes (1,2). ARID5A expression is seen in many immune cells, including macrophages, CD4 T cell subsets TH1 and TH17, as well as stromal cells and keratinocytes (2). During inflammation, ARID5A is translocated from the nucleus to the cytoplasm and stabilizes a variety of mRNA transcripts, including IL-6, Stat3, OX40, T-bet, and IL-17, contributing to the inflammatory response and a variety of inflammatory diseases. TLR4-activated NF-kB and MAPK pathways are involved in regulating ARID5A expression (3). ARID5A plays an important role in resistance to chimeric antigen receptor (CAR)-T cell therapy by stabilizing IDO1 mRNA and the upregulation of IDO1 expression. Elevated IDO1 expression levels facilitate the conversion of tryptophan to kynurenine, which contributes to CAR-T cell exhaustion (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARI5A; ARID domain-containing protein 5A; Arid5a; AT rich interactive domain 5A (Mrf1 like); AT rich interactive domain 5A (MRF1-like); AT-rich interactive domain-containing protein 5A; D430024K22Rik; MGC36895; modulator recognition factor I; Mrf; Mrf1; OTTMUSP00000023948\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800100262057,"sku":"49054T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-49054t","provider":"Iright","version":"1.0","type":"link"}