{"product_id":"cst-49316s","title":"CST,  49316S, OCA-T1 (E4T1K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying OCA-T1\/POU2AF2\/C11orf53. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, OCA-T1 (E4T1K) Rabbit Monoclonal Antibody (CST #49316) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78954 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOCA-T1 (E4T1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total OCA-T1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro150 of human OCA-T1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nOCA-T1 is encoded by the gene ( ), which was identified in a single-cell RNA sequencing study to be expressed selectively in tuft cells from mouse and human tissues (1). The expression of OCA-T1 was observed to occur in a pattern strikingly similar to that of POU2F3, a master regulator of tuft cell development whose expression also defines a tuft cell-like variant (SCLC-P) of small cell lung cancers (2). Although containing no recognizable structural domains, OCA-T1 showed minor sequence similarity with OCA-B, a protein encoded by the gene and reported to be an activator of selected Class II POU domain transcription factors (3). Functional studies revealed that OCA-T1 forms a protein complex with POU2F3; these proteins furthermore share genomic binding sites and promote the expression of genes directly associated with tuft cell development. Lastly, Crispr\/Cas9-mediated deletion of in mice revealed that OCA-T1 is functionally required for normal tuft cell development (1). Collectively, these findings suggest that OCA-T1 is a critical component of the POU2F3-containing transcriptional complex that regulates the expression of genes governing tuft cell development.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC11orf53; CK053; MGC50104; OCA-T1; POU class 2 homeobox associating factor 2; POU class 2 homeobox-associating factor 2; POU domain class 2-associating factor 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799691841705,"sku":"49316S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-49316s","provider":"Iright","version":"1.0","type":"link"}