{"product_id":"cst-4936s","title":"CST,  4936S, Wee1 Antibody","description":"Polyclonal Antibody for studying Wee1. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Wee1 Antibody (CST #4936) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nWee1 Antibody detects endogenous levels of Wee1 protein independent of phosphorylation.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human Wee1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nEntry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2\/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7). Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by beta-TrCP-mediated ubiquitination and degradation (1,9,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp686I18166; FLJ16446; WEE1; WEE1 G2 checkpoint kinase; WEE1 homolog; Wee1-like protein kinase; WEE1+ homolog; WEE1A; Wee1A kinase; WEE1hu\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799696199849,"sku":"4936S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4936s","provider":"Iright","version":"1.0","type":"link"}