{"product_id":"cst-4941t","title":"CST,  4941T, Phospho-PBK\/TOPK (Thr9) Antibody","description":"Polyclonal Antibody for studying PBK\/TOPK (Thr9) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PBK\/TOPK (Thr9) Antibody (CST #4941) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PBK\/TOPK (Thr9) Antibody detects endogenous levels of PBK\/TOPK only when phosphorylated at threonine 9.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Thr9 of human PBK\/TOPK. Antibodies are purified by protein A and affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nPBK\/TOPK is a serine\/threonine kinase that is phosphorylated and active during mitosis (1). PBK\/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-binding domain, which is thought to interact with the human homologue of the Drosophila Discs large (hDlg) tumor suppressor protein (1). Increased PBK\/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK\/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK\/TOPK has been observed (4), and cdc2\/cyclin B has been shown to phosphorylate PBK\/TOPK , presumably at Thr9 (5). Potential substrates of PBK\/TOPK include p38 MAPK and c-Myc (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCancer\/testis antigen 84; CT84; epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PBK; PDZ binding kinase; PDZ-binding kinase; serine\/threonine protein kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799702065321,"sku":"4941T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4941t","provider":"Iright","version":"1.0","type":"link"}