{"product_id":"cst-4951s","title":"CST,  4951S, Phospho-KSR1 (Ser392) Antibody","description":"Polyclonal Antibody for studying KSR1 (Ser404) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-KSR1 (Ser392) Antibody (CST #4951) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-KSR1 (Ser392) Antibody detects endogenous levels of KSR1 only when phosphorylated at serine 392. This antibody may also react with phosphorylated KSR2 at the equivalent site.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser392 of mouse KSR1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nKSR1 (kinase supressor of Ras) was identified from a genetic screen in and as a component of the Ras signaling pathway (1). KSR1 has a putative carboxy-terminal kinase domain that lacks a key Lys residue for phospho-group transfer. Although reports indicate that ceramide and EGF activate KSR1 (2,3), other evidence demonstrates that KSR1 regulates Raf in a kinase-independent manner (4,5). It is now widely accepted that KSR1 functions as a scaffold that binds MEK1\/2 and 14-3-3 protein constitutively and binds ERK1\/2 in a Ras activation-dependent manner (1,5,6). HSP70\/HSP90 and p50 Cdc37 associate with the KSR1 complex to ensure its stability (5). Multiple phosphorylation sites have been identified: Ser297 and Ser392 mediate 14-3-3 binding, and putative MAPK phosphorylation sites include Thr260, Thr274 and Ser443 (6). C-TAK1 (Cdc25C-associated kinase 1) binds and phosphorylates KSR1 at Ser392 in quiescent cells (7). In response to stimuli, Ser392 is dephosphorylated by PP2A, which leads to ERK1\/2 association and allows the KSR1 complex to translocate from cytosol to membrane, where the MAPK pathway is activated (8). IMP, a Ras-responsive E3 ubiquitin ligase, is also involved in interaction with KSR1 and may regulate its localization and stability (9). Very high expression levels of KSR1 inhibit MAPK signaling, whereas physiological levels promote MAPK signaling, indicating that the scaffold protein can turn signaling \"on\" or \"off\" depending on the scaffold concentration (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nkinase suppressor of ras; Kinase suppressor of Ras 1; KSR; KSR1; RSU2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 115\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799712321705,"sku":"4951S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4951s","provider":"Iright","version":"1.0","type":"link"}