{"product_id":"cst-49747s","title":"CST,  49747S, DUSP3\/VHR (F5R7B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying VHR\/DUSP3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DUSP3\/VHR (F5R7B) Rabbit Monoclonal Antibody (CST #49747) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDUSP3\/VHR (F5R7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total DUSP3\/VHR protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of DUSP3\/VHR, but is not expected to detect other DUSP proteins. This antibody detects an approximately 25 kDa protein of unknown identity in some cell lines.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro162 of human DUSP3\/VHR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDual specificity protein phosphatase 3 (DUSP3), also known as Vaccinia H1-related phosphatase (VHR), is a small dual-specific phosphatase with specificity for MAP kinase ERK1\/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen-stimulated T cells, DUSP3 is phosphorylated by Zap-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to downregulate the ERK and JNK pathways (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ndual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3; DUSP3; serine\/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 20\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799725527209,"sku":"49747S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-49747s","provider":"Iright","version":"1.0","type":"link"}