{"product_id":"cst-4997t","title":"CST,  4997T, UBC3 Antibody","description":"Polyclonal Antibody for studying UBC3B\/UBC3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, UBC3 Antibody (CST #4997) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUBC3 Antibody detects endogenous levels of total UBC3 and UBC3B protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human UBC3. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein\/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein\/b-Trcp) complex mediates degradation of b-catenin (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n(E3-independent) E2 ubiquitin-conjugating enzyme R1; CDC34; CDC34B; cell division cycle 34 homolog; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34, ubiqiutin conjugating enzyme; E2 ubiquitin-conjugating enzyme R1; E2 ubiquitin-conjugating enzyme R2; E2-CDC34; E2-CDC34B; FLJ20419; MGC10481; UB2R1; UB2R2; UBC3; UBC3B; UBCH3; UBE2R1; UBE2R2; ubiquitin carrier protein; Ubiquitin carrier protein R2; ubiquitin conjugating enzyme E2 R2; ubiquitin conjugating enzyme E2R 2; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B; Ubiquitin-protein ligase R1; Ubiquitin-protein ligase R2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799738962089,"sku":"4997T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4997t","provider":"Iright","version":"1.0","type":"link"}