{"product_id":"cst-50049s","title":"CST,  50049S, CaMKII-alpha (6G9) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying CaMK2-alpha. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CaMKII-alpha (6G9) Mouse Monoclonal Antibody (CST #50049) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92284 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCaMKII-alpha (6G9) Mouse Monoclonal Antibody recognizes endogenous levels of total CaMKII-α protein. This antibody does not cross-react with CaMKII-β or CaMKII-δ proteins.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a partially purified rat CaMKII-α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaMKII is an important member of the calcium\/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca \/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1\/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncalcium\/calmodulin dependent protein kinase II alpha; calcium\/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium\/calmodulin-dependent protein kinase II; calcium\/calmodulin-dependent protein kinase II alpha; calcium\/calmodulin-dependent protein kinase II alpha-B subunit; calcium\/calmodulin-dependent protein kinase type II alpha chain; Calcium\/calmodulin-dependent protein kinase type II subunit alpha; calmodulin-dependent kinase II; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMK-II subunit alpha; CAMK2A; CAMKA; CaMKII; CaMKIIalpha; CaMKIINalpha; KCC2A; KIAA0968; MRD53; MRT63\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799745155241,"sku":"50049S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50049s","provider":"Iright","version":"1.0","type":"link"}