{"product_id":"cst-5026s","title":"CST,  5026S, Phospho-Myosin IIa (Ser1943) Antibody","description":"Polyclonal Antibody for studying Myosin IIa (Ser1943) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Myosin IIa (Ser1943) Antibody (CST #5026) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Myosin IIa (Ser1943) Antibody detects endogenous levels of myosin IIa protein only when phosphorylated at Ser1943.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1943 of human myosin IIa protein. Antibodies are purified using protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nNonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, , , and , which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBDPLT6; Cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MGC104539; MHA; MYH9; MYH9 variant protein; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Myosin-9; myosin, heavy chain 9, non-muscle; NMHC-II-A; NMMHC II-a; NMMHC-A; NMMHC-IIA; NMMHCA; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 230\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799753904297,"sku":"5026S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5026s","provider":"Iright","version":"1.0","type":"link"}