{"product_id":"cst-50273s","title":"CST,  50273S, COL4A1 Antibody","description":"Polyclonal Antibody for studying COL4A1. Validated for Western Blotting. Highly specific and rigorously validated in-house, COL4A1 Antibody (CST #50273) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCOL4A1 Antibody recognizes endogenous levels of total COL4A1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human COL4A1 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nType IV collagens support extracellular matrix (ECM) function by forming a cross-linking network in the basement membrane zone. There are six different type IV collagens that are encoded by the following genes: , , , , , and . Among them, COL4A1 and COL4A2 are the most common collagens found in the basement membrane, and are usually used as a marker of the basement membrane dynamic stability (1). The type IV collagen fiber exists as a trimeric complex composed of two molecules of COL4A1 and one molecule of COL4A2. The N-terminal 7S domains of the protein function on intermolecular cross-linking, and C-terminal non-collagenous domains (NCI) are responsible for heterotrimer assembly. The trimeric fiber further associates to form a cross-linked sheet structure by NCI\/NCI and 7S\/7S interaction (2). COL4A1 plays a critical role in maintaining basement membrane integrity for normal tissue functions. Genetic mutations of COL4A1 and COL4A2 have been linked to cardiovascular disease, cerebral small vessel disease, and glomerulocystic kidney disease (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nArresten; BSVD; BSVD1; CO4A1; COL4A1; COL4A1 NC1 domain; COL4A1s; Collagen 4; Collagen alpha-1(IV) chain; collagen IV; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen type IV; collagen type IV alpha 1 chain; collagen, type IV, alpha 1; PADMAL; RATOR; type IV collagen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799755313321,"sku":"50273S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50273s","provider":"Iright","version":"1.0","type":"link"}