{"product_id":"cst-50311s","title":"CST,  50311S, EPLIN (D1A7A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying eplin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, EPLIN (D1A7A) Rabbit Monoclonal Antibody (CST #50311) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEPLIN (D1A7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total EPLIN protein, including EPLIN-α and EPLIN-β isoforms. Based on the absence of signal in NCI-H28 cells, which appear to express low levels of β-Eplin, this antibody may have a preference for α-Eplin by immunofluorescence.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly455 of human EPLIN protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpithelial Protein Lost in Neoplasm (EPLIN) is an actin-binding protein that regulates actin filament dynamics and cross-linking (1). Alpha and beta isoforms are generated from alternate promoters, with the EPLIN-β isoform representing the full-length protein and the EPLIN-α isoform lacking the amino-terminal 160 amino acids (2). Increased expression of EPLIN protein results in more abundant and larger actin stress fibers due to stabilizing of cross-links and inhibition of actin depolymerization. EPLIN protein inhibits Rac1-promoted membrane ruffling and Arp2\/3-associated actin filament branching (1). Research studies demonstrate reduced EPLIN-α expression in tumor tissues, and correlate this reduction with increased invasiveness and poor clinical outcomes (3). The EPLIN protein is an important negative regulator of the epithelial-mesenchymal transition (EMT)(4). While EMT is a critical process during normal embryonic development, dysregulation in transformed cells is a key step in the transition to metastasis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n1110021C24Rik; Epithelial protein lost in neoplasm; epithelial protein lost in neoplasm beta; EPLIN; FLJ38853; LDLCQ8; LIM domain and actin binding 1; LIM domain and actin-binding protein 1; LIMA1; MGC131726; SREBP3; sterol regulatory element binding protein 3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 85, 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799760130217,"sku":"50311S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50311s","provider":"Iright","version":"1.0","type":"link"}