{"product_id":"cst-50396sf","title":"CST,  50396SF, Phospho-FAK (Tyr397) (D20B1) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying FAK (Tyr397) phosphate. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-FAK (Tyr397) (D20B1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #50396) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #8556. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8556\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-FAK (Tyr397) (D20B1) Rabbit mAb (BSA and Azide Free) detects FAK protein only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFocal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFADK; FADK 1; FAK; FAK-related non-kinase polypeptide; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 125\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799764914345,"sku":"50396SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50396sf","provider":"Iright","version":"1.0","type":"link"}