{"product_id":"cst-50402s","title":"CST,  50402S, DDX1 (F5C8A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DDX1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX1 (F5C8A) Rabbit Monoclonal Antibody (CST #50402) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDDX1 (F5C8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX1 protein. This antibody detects a 52 kDa protein of unknown identity in some cell lines.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly686 of human DDX1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDead box protein 1 (DDX1) is a DEAD box RNA helicase that is involved in 3'-end processing of pre-mRNA. DDX1 is capable of binding to poly(A) RNA and interacts with several key RNA-binding proteins, including hnRNP K, and forms discrete nuclear foci with the CSTF complex called DDX1 bodies (1,2). During DNA damage, DDX1 is recruited to double-strand breaks (DSBs) upon phosphorylation by ATM, where it plays a role in RNA clearance at the DSB sites (3). DDX1 phosphorylation has also been shown to increase expression of a subset of microRNAs (miRNAs) that can inhibit tumor progression (4). DDX1 has been shown to bind to the HIV-1 Rev protein, and DDX1 downregulation disrupts viral production (5,6). Additionally, DDX1 has been implicated in other viral infections, including SARS-CoV-2, where DDX1 binds the nucleocapsid protein to inhibit its function (7). DDX1 has been described in various cancer types, but notably, the gene is co-amplified with in neuroblastoma (8-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATP-dependent RNA helicase DDX1; DBP-RB; DDX1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD-box helicase 1; DEAD\/H (Asp-Glu-Ala-Asp\/His) box polypeptide 1; DEAD\/H-box helicase 1; UKVH5d\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799765897385,"sku":"50402S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50402s","provider":"Iright","version":"1.0","type":"link"}