{"product_id":"cst-50607t","title":"CST,  50607T, FTSJ3 (E6T9J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FTSJ3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FTSJ3 (E6T9J) Rabbit Monoclonal Antibody (CST #50607) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFTSJ3 (E6T9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total FTSJ3 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFTSJ3 is an RNA 2'-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2'-O-methylation at the 5'-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2'-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2'-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2'-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2'-O-ribose RNA methyltransferase SPB1 homolog; EPCS3; FLJ20062; FtsJ homolog 3; FtsJ homolog 3 (E. coli); FtsJ RNA 2'-O-methyltransferase 3; FtsJ RNA methyltransferase homolog 3; FTSJ3; pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3; pre-rRNA processing protein FTSJ3; Protein ftsJ homolog 3; Putative rRNA methyltransferase 3; RRMJ3; rRNA (uridine-2'-O-)-methyltransferase 3; SPB1; SPB1 RNA methyltransferase homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799779168425,"sku":"50607T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50607t","provider":"Iright","version":"1.0","type":"link"}