{"product_id":"cst-50616t","title":"CST,  50616T, SGO2 (E9K4N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Sgo2. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SGO2 (E9K4N) Rabbit Monoclonal Antibody (CST #50616) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:3200 - 1:12800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84360 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSGO2 (E9K4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SGO2 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize isoforms 1-3 of human SGO2 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly15 of human SGO2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSGO2 (Shugoshin-2, SGOL2) is a centromeric protein required for the protection of cohesion complexes in meiosis (1). In mitosis, SGO2 and MAD2 regulate separase activity in the absence of securin to ensure appropriate timing of chromosome segregation (2). SGO2 is highly expressed in prostate cancer, and it promotes proliferation and migration by stabilizing the small GTPase Rab1A (3). High SGO2 expression in hepatocellular carcinoma (HCC) predicts poor overall survival (4), promoting tumor proliferation by regulating MAD2 expression and function (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ25211; MGC129665; Sgo2; SGOL2; Shugoshin 2; Shugoshin-2; Shugoshin-like 2; shugoshin-like 2 (S. pombe); Tripin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 190\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799779266729,"sku":"50616T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50616t","provider":"Iright","version":"1.0","type":"link"}