{"product_id":"cst-50708s","title":"CST,  50708S, HCFC1 Antibody (Carboxy-terminal Antigen)","description":"Polyclonal Antibody for studying HCFC1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, HCFC1 Antibody (Carboxy-terminal Antigen) (CST #50708) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHCFC1 Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total HCFC1 protein. This antibody also recognizes carboxyl terminal fragments (HCFC1-C) resulting from O-GlcNAc transferase (OGT) cleavage.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu1590 of human HCFC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nHost cell factor C1 (HCFC1) was first identified as the host cell factor for human herpes simplex virus infection. HCFC1 and the viral protein VP16 belong to a multi-protein complex that promotes transcription of viral immediate early genes (1). The relatively large HCFC1 protein contains 6 centrally located 26 amino acid repeats that can be O-GlcNAcylated and subjected to O-linked beta-N-acetylglucosamine transferase (OGT) cleavage (2-4). The resulting amino-terminal (HCFC1-N) and carboxy-terminal (HCFC1-C) fragments are non-covalently associated and play important roles in cell cycle regulation. The HCFC1-N peptide facilitates progression through the G1 phase of the cell cycle while HCFC1-C enables proper mitosis and cytokinesis during the M phase (5-7). As HCFC1 plays an important role in neurodevelopment, mutations in the corresponding gene are associated with neurodevelopmental disorders (e.g., intellectual disability) in humans (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC1 factor; CFF; HCF; HCF C-terminal chain 1; HCF C-terminal chain 2; HCF C-terminal chain 3; HCF C-terminal chain 4; HCF C-terminal chain 5; HCF C-terminal chain 6; HCF N-terminal chain 1; HCF N-terminal chain 2; HCF N-terminal chain 3; HCF N-terminal chain 4; HCF N-terminal chain 5; HCF N-terminal chain 6; HCF-1; HCF1; HCFC1; HFC1; Host cell factor 1; host cell factor C1; host cell factor C1 (VP16-accessory protein); MGC70925; MRX3; PPP1R89; protein phosphatase 1, regulatory subunit 89; VCAF; VP16 accessory protein; VP16-accessory protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 100, 125, 130, 145, 260\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799779725481,"sku":"50708S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50708s","provider":"Iright","version":"1.0","type":"link"}