{"product_id":"cst-50794t","title":"CST,  50794T, IL-1 alpha (D4F3S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IL1 alpha mouse. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, IL-1 alpha (D4F3S) Rabbit Monoclonal Antibody (CST #50794) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-1 alpha (D4F3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-1α protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse IL-1α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key \"alarmin in the cell\" that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIL-1 alpha; Il-1a; Il1a; interleukin 1 alpha; Interleukin-1 alpha; OTTMUSP00000016430\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800101015721,"sku":"50794T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50794t","provider":"Iright","version":"1.0","type":"link"}