{"product_id":"cst-50805s","title":"CST,  50805S, SET1A (E3E2S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SETD1A mouse. Validated for WB,IP,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, SET1A (E3E2S) Rabbit Monoclonal Antibody (CST #50805) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-Seq results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nChromatin IP: 1:25\nChromatin IP-seq: 1:25\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSET1A (E3E2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SET1A protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse SET1A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Set1 histone methyltransferase protein was first identified in yeast as part of the Set1\/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1\/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBC010250; Histone-lysine N-methyltransferase; Histone-lysine N-methyltransferase SETD1A; KMT2F; MGC55143; mKIAA0339; mNSC1; non-selective cation channel 1; Nsccn1; OTTMUSP00000018899; SET domain containing 1A; SET domain-containing protein 1A; SET1A; Setd1a\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799780217001,"sku":"50805S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50805s","provider":"Iright","version":"1.0","type":"link"}