{"product_id":"cst-50830s","title":"CST,  50830S, GHF-1 (F9C3D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GHF-1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, GHF-1 (F9C3D) Rabbit Monoclonal Antibody (CST #50830) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGHF-1 (F9C3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total GHF-1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu86 of human GHF-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGrowth hormone factor 1 (GHF-1), also known as Pit-1, is a POU domain transcription factor essential for the development and function of specific anterior pituitary cells (i.e., somatotrophs, lactotrophs, and thyrotrophs) (2). It activates the genes for growth hormone, prolactin, and TSH beta, crucial for hormone production and pituitary cell lineage determination (1,2). Mutations in (the gene encoding GHF-1) are a major cause of combined pituitary hormone deficiency and isolated growth hormone deficiency, leading to impaired hormone secretion and developmental issues (3). While GHF-1 is crucial for normal pituitary development, its role in pituitary tumorigenesis is complex and not fully elucidated. Some pituitary adenomas, particularly those of the somatotroph and lactotroph lineages, express GHF-1. However, mutations are not a common cause of sporadic pituitary adenomas. Aberrant GHF-1 expression or activity might contribute to tumor progression in some cases, but further research is needed (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCPHD1; GHF-1; GHF1; Growth hormone factor 1; PIT-1; PIT1; pituitary transcript factor 1; Pituitary-specific positive transcription factor 1; pituitary-specific transcription factor 1; POU class 1 homeobox 1; POU domain, class 1, transcription factor 1; POU1F1; POU1F1a\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 29\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799780479145,"sku":"50830S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50830s","provider":"Iright","version":"1.0","type":"link"}