{"product_id":"cst-50850t","title":"CST,  50850T, TWIST1 (E2M5V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TWIST1. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TWIST1 (E2M5V) Rabbit Monoclonal Antibody (CST #50850) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunohistochemistry (Paraffin): 1:250 - 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTWIST1 (E2M5V) Rabbit Monoclonal Antibody recognizes endogenous levels of total TWIST1 protein. Non-specific cytoplasmic staining was observed in mouse brain by immunohistochemistry.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly35 of human TWIST1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTWIST1 is a basic helix-loop-helix (BHLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the BHLH domain of the gene have been associated with Saethre-Chotzen syndrome (SCS), an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in epithelial-mesenchymal transition (EMT) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to taxol and microtubule-regulating drugs in tumors (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nACS3; B-HLH DNA binding protein; BHLHA38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; SCS; SWCOS; TWIST; twist basic helix-loop-helix transcription factor 1; twist family bHLH transcription factor 1; twist homolog 1; twist homolog 1 (Drosophila); TWIST homolog of drosophila; Twist-related protein 1; TWIST1; TWST1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800283205801,"sku":"50850T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-50850t","provider":"Iright","version":"1.0","type":"link"}