{"product_id":"cst-5147s","title":"CST,  5147S, SRF (D71A9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SRF. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SRF (D71A9) Rabbit Monoclonal Antibody (CST #5147) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSRF (D71A9) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRF protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser375 of human SRF protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc-fos serum response element-binding transcription factor; MCM1; minichromosome maintenance 1 homolog; Serum response factor; serum response factor (c-fos serum response element-binding transcription factor); SRF\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Pg\nSENSITIVITY: Endogenous\nMW (kDa): 67\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799784640681,"sku":"5147S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5147s","provider":"Iright","version":"1.0","type":"link"}