{"product_id":"cst-5153t","title":"CST,  5153T, Androgen Receptor (D6F11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Androgen Receptor. Validated for WB,WB,IP,IHC,IF,F,ChIP,ChIP,C\u0026amp;R. Available in 3 sizes. Highly specific and rigorously validated in-house, Androgen Receptor (D6F11) Rabbit Monoclonal Antibody (CST #5153) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using the CUT\u0026amp;RUN Assay Kit #CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:2000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:600 - 1:1200\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:800\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 23791 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAndrogen Receptor (D6F11) Rabbit Monoclonal Antibody detects endogenous levels of total androgen receptor protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAndrogen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIS; ANDR; Androgen receptor; AR; AR8; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; TFM\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799784837289,"sku":"5153T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5153t","provider":"Iright","version":"1.0","type":"link"}