{"product_id":"cst-51550s","title":"CST,  51550S, Tryptase (E9R8A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Tryptase. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Tryptase (E9R8A) Rabbit Monoclonal Antibody (CST #51550) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTryptase (E9R8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tryptase protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to human Tryptase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells (1). Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent) (2). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin (3-5). Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides (6), calcitonin gene-related peptides (7), fibronectins (8), fibrinogens (3), and kininogens (9). Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts (10-13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nepididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; TPS1; TPS2; TPSAB1; TPSB1; TPSB2; TRYA1; TRYB1; tryptase alpha II; Tryptase alpha-1; tryptase alpha\/beta 1; Tryptase alpha\/beta-1; tryptase beta 1; tryptase beta I; tryptase beta-1; Tryptase beta-2; tryptase I; Tryptase II; Tryptase-1; Tryptase-2; tryptase-I; tryptase-III\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 25-40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799785328809,"sku":"51550S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-51550s","provider":"Iright","version":"1.0","type":"link"}