{"product_id":"cst-51613s","title":"CST,  51613S, MED1 Antibody","description":"Polyclonal Antibody for studying MED1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MED1 Antibody (CST #51613) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMED1 Antibody recognizes endogenous levels of total MED1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His591 of human MED1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops' that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter and works to sustain proper chromatin architecture during active transcription (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; MGC71488; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARG binding protein; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRAP220; TRIP-2; TRIP2; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 220\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799785885865,"sku":"51613S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-51613s","provider":"Iright","version":"1.0","type":"link"}