{"product_id":"cst-51886t","title":"CST,  51886T, Granzyme F (E2U7L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Granzyme F mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme F (E2U7L) Rabbit Monoclonal Antibody (CST #51886) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Frozen): 1:100 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 30530 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Frozen), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGranzyme F (E2U7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme F protein. This antibody does not cross-react with other Granzyme proteins.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln197 of mouse Granzyme F protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGranzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme F induces a novel cell death pathway consisting of externalization of phosphatidylserine (PS), nuclear condensation, mitochondrial damage, cytochrome c release, single-stranded DNA nicking, and caspase inactivation (4). CTLs from mice with reduced expression of Granzyme F and C display diminished cytotoxicity (5). Â\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAKA granzyme G; C134; CCP; CCP4; Ctl; CTL serine protease 3; Ctla; Ctla-7; Ctla7; Cytotoxic cell protease 4; Cytotoxic serine protease 3; GRAF; Granzyme F; granzyme G; Gzmf; GZMH; MCSP; MCSP-3; MCSP3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 30-40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800101572777,"sku":"51886T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-51886t","provider":"Iright","version":"1.0","type":"link"}